Our Lady of Guadalupe Parish is a Roman Catholic parish in Madison, Wisconsin, part of the Lake Mendota Vicariate.

Our Lady of Guadalupe Parish is a Roman Catholic parish located in Madison, Wisconsin. It belongs to the Lake Mendota Vicariate within the Roman Catholic Diocese of Madison, which also includes Our Lady Queen of Peace Parish, St. Thomas Aquinas Parish, Queen of Apostles Parish, Blessed Sacrament Parish, St. Paul University Parish, and the Cathedral of St. Bernard of Clairvaux. The vicariate structure groups parishes regionally to coordinate pastoral action, and the Lake Mendota Vicariate is overseen by a vicar forane, currently Fr. Brian Wilk, who serves as pastor of Our Lady Queen of Peace Parish. As a parish in this vicariate, Our Lady of Guadalupe participates in collaborative efforts with neighboring parishes to serve the Catholic community in the Madison area.